Optimisation of PCR reactions using primer chessboarding
نویسندگان
چکیده
منابع مشابه
Optimisation of PCR reactions using primer chessboarding.
In-house polymerase chain reaction (PCR) assays are now an integral part of the work of most diagnostic microbiological laboratories. Despite the availability of commercial reagent 'master-mixes' of some PCR reagents, the optimisation of primers still poses a significant problem. Here, we describe a simple method to assess the concentration of primer needed in single round, multiplex, nested an...
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ژورنال
عنوان ژورنال: Journal of Clinical Virology
سال: 2003
ISSN: 1386-6532
DOI: 10.1016/s1386-6532(03)00006-4